Contribution of Flavin Covalent Linkage with Histidine 99 to the Reaction Catalyzed by Choline Oxidase
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چکیده
منابع مشابه
Roles of Serine 101, Histidine 310 and Valine 464 in the Reaction Catalyzed by Choline Oxidase from Arthrobacter Globiformis
The enzymatic oxidation of choline to glycine betaine is of interest because organisms accumulate glycine betaine intracellularly in response to stress conditions, as such it is of potential interest for the genetic engineering of crops that do not naturally possess efficient pathways for the synthesis of glycine betaine, and for the potential development of drugs that target the glycine betain...
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The flavin-dependent enzyme 2-haloacrylate hydratase (2-HAH) catalyzes the conversion of 2-chloroacrylate, a major component in the manufacture of acrylic polymers, to pyruvate. The enzyme was expressed in Escherichia coli, purified, and characterized. 2-HAH was shown to be monomeric in solution and contained a non-covalent, yet tightly bound, flavin adenine dinucleotide (FAD). Although the cat...
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Choline oxidase catalyzes the two-step oxidation of choline to glycine betaine, one of limited osmoprotectants, with the formation of betaine aldehyde as an enzyme bound intermediate. Glycine betaine accumulates in the cytoplasm of plants and bacteria as a defensive mechanism to withstand hyperosmolarity and elevated temperatures. This makes the genetic engineering of relevant plants which lack...
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Introduction Since its discovery, D-amino acid oxidase (EC 1.4.3.3, DAAO) has received much attention both from the physiological and biochemical points ofview. The groups of Massey (USA), Curti (1taly), and Yagi (Japan) were mainly involved in its study over the year (1). In spite of these efforts the basic molecular mechanism underlying the reductive half-reaction, i.e. the dehydrogenation st...
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loxiazine ring. Effect of pH on fluorescence of the flavin peptide was shown in fig. 1. The fluorescence of the flavin peptide was quenched at neutral and alkaline pH ranges. This shows that the amino acid linked to flavin ring is a histidyl residue.6) The pKa. value of the flavin peptide was found to be about 4.6, which was almost identical with that of the authentic 8a-[7V(3)-histidyl]ribofla...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2009
ISSN: 0021-9258
DOI: 10.1074/jbc.m109.003715